Insulin-like androgenic gland hormone (IAG) secreted by a crustacean male-specific androgenic gland (AG) is regarded as a peptidergic hormone and regulates male sex differentiation. They are classified into type-I and type-II peptides, involved in inhibiting ecdysteroid, methyl farnesoate, and vitellogenin synthesis ( 1). Crustacean hyperglycemic hormone superfamily (CHHs) are the typical crustacean peptide hormones. Peptide hormones play an important role in crustaceans reproduction. Overall, the first study on identification of peptides and their GPCRs in the genus Lysmata extends our knowledge of peptidergic signaling in PSH species, and provides an important basis for development of aquaculture strategies. Among them, insulin-like androgenic gland hormone and crustacean female sex hormone have two unique mature peptides, respectively, and their transcripts showed higher expression levels in MP than EP, which suggest that these sex differentiation hormones might be involved in sexual characters than spermatogenesis or vitellogenesis. A total of 61 peptide and 40 peptide GPCR transcripts derive from 44 peptide families and 13 peptide GPCR families were identified, respectively. This study employed Illumina sequencing and bioinformatics analysis to identify peptides and their candidate GPCRs from male phase (MP) and euhermaphrodite phase (EP) of L. Knowledge on reproductive biology of this commercial species is critical for resources management and aquaculture. The peppermint shrimp Lysmata vittata popular in marine ornamental trade and is known to display protandric simultaneous hermaphrodite (PSH). Peptide hormones commonly binding with G-protein coupled receptors (GPCRs) achieve their function in reproduction.
0 kommentar(er)
